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KMID : 0545120220320080982
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Journal of Microbiology and Biotechnology
2022 Volume.32 No. 8 p.982 ~ p.988
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Dehydroglyasperin D Suppresses Melanin Synthesis through MITF Degradation in Melanocytes
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Baek Eun-Ji
Ha Yu-Bin
Kim Ji-Hye
Lee Ki-Won
Lim Soon-Sung
Kang Nam-Joo
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Abstract
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Licorice (Glycyrrhiza) has been used as preventive and therapeutic material for hyperpigmentation disorders. Previously, we isolated noble compounds including dehydroglyasperin C (DGC), dehydroglyasperin D (DGD) and isoangustone A (IAA) from licorice hexane/ethanol extracts. However, their anti-melanogenic effects and underlying molecular mechanisms are unknown. The present study compared effects of DGC, DGD and IAA on pigmentation in melan-a melanocytes and human epidermal melanocytes (HEMn). DGD exerted the most excellent anti-melanogenic effect, followed by DGC and IAA at non-cytotoxic concentrations. In addition, DGD significantly inhibited tyrosinase activity in vitro cell-free system and cell system. Western blot result showed that DGD decreased expression of microphthalmia-associated transcription factor (MITF), tyrosinase and tyrosinase-related protein-1 (TRP-1) in melan-a cells and HEMn cells. DGD induced phosphorylation of MITF, ERK and Akt signal pathway promoting MITF degradation system. However, DGD did not influence p38 and cAMP-dependent protein kinase (PKA)/CREB signal pathway in melan-a cells. These result indicated that DGD inhibited melanogenesis not only direct regulation of tyrosinase but also modulating intracellular signaling related with MITF level. Collectively, these results suggested a protective role for DGD against melanogenesis.
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KEYWORD
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Licorice, dehydroglyasperin D, melanin, microphthalmia-associated transcription factor, melanocytes
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